Quality Control on Salmonella Shigella Agar coli, mucoid, pale, opaque cream to pink. They do not produce any hydrogen sulfide.Įnterobacter and Klebsiella appears larger than E. coli will ferment the lactose in the media, resulting in bacterial growth with a pink color. Shigella do not ferment lactose or produce hydrogen sulfide gas, so the resulting colonies will be colorless.Ĭoliform bacteria such as E. The resulting bacterial colonies will appear colorless with black centers. Salmonella will not ferment lactose, but produce hydrogen sulfide (H 2S) gas. Result Interpretation on Salmonella Shigella Agar Mix well and pour into sterile Petri plates.Heat to boiling to dissolve the medium completely.Suspend 60.0 grams of Salmonella Shigella Agar in 1000 ml distilled water.It was also developed to aid in the differentiation of lactose and non-lactose-fermenters from clinical specimens, suspected foods, and other such samples.This medium is not recommended for the primary isolation of Shigella.It is used as a selective and differential medium for the isolation of Salmonella and some Shigella species from clinical and non-clinical specimens.Neutral red turns red in the presence of an acidic pH, thus showing fermentation has occurred. Thiosulfate and Ferric Citrate permit detection of hydrogen sulfide by the production of colonies with black centers. Lactose is the carbohydrate present in Salmonella Shigella Agar. Beef Extract, Enzymatic Digest of Casein, and Enzymatic Digest of Animal Tissue provide sources of nitrogen, carbon, and vitamins required for organism growth. The inclusion of Bile Salts, Sodium Citrate and Brilliant Green serve to inhibit gram-positive, coliform organisms and inhibit swarming Proteus spp., while allowing Salmonella spp. PH ( at 25☌) 7.0 ± 0.2 Principle of Salmonella Shigella Agar Composition of Salmonella Shigella Agar Ingredients It is recommended for testing clinical specimens and food testing for the presence of Salmonella spp. SS Agar is a modification of the Desoxycholate Citrate Agar. Salmonella Shigella (SS) Agar is moderately selective and differential medium for the isolation, cultivation and differentiation of Salmonella spp.
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